天天做天天干,国产极品粉嫩小泬免费看,国产,各种自啪精品视频,五月婷婷丁香狠

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>小鼠腫瘤細(xì)胞、癌細(xì)胞>CRL-1838M-NFS-60 小鼠白血病細(xì)胞G-CSF依賴性

M-NFS-60 小鼠白血病細(xì)胞G-CSF依賴性

簡(jiǎn)要描述:CRL-1838 M-NFS-60 小鼠白血病細(xì)胞G-CSF依賴性,
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件,

  • 產(chǎn)品型號(hào):CRL-1838
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-09-11
  • 訪  問(wèn)  量:2917

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1838 M-NFS-60 小鼠白血病細(xì)胞G-CSF依賴性, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件,

CRL-1838 M-NFS-60 小鼠白血病細(xì)胞G-CSF依賴性 的詳細(xì)介紹

CRL-1838

ATCC® Number:CRL-1838™  Price:$349.00
Designations:M-NFS-60

Depositors:JF Weaver, A Creasey

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:suspension

Organism:Mus musculus (mouse)

Morphology:lymphoblast


Source:Disease: myelogenous leukemia
Cell Type: virus induced


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Applications:transfection host (Roche FuGENE® Transfection Reagents)

Comments:The M-NFS-60 cell line was derived from a myelogenous leukemia induced with the Cas-Br-MuLV wild mouse ecotropic retrovirus.

The cells are responsive to both interleukin 3 (interleukin-3, IL-3) and macrophage colony stimulating factor (M-CSF).

The cells contain a truncated c-myb proto - oncogene caused by integration of a retrovirus.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • 0.05 mM 2-mercaptoethanol

  • 62 ng/ml human recombinant macrophage colony stimulating factor (M-CSF)

  • fetal bovine serum to a final concentration of 10%.
    Temperature: 37.0°C



Subculturing:Medium Renewal: Add fresh medium at the time of subculture

Cultures can be maintained by addition or replacement of fresh medium. Subcuture every two days at 2.5 X 10 exp4 viable cells/ml.



Preservation:culture medium 95%; DMSO, 5%

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020



References:22842: Nakoinz I, et al. Differentiation of the IL-3-dependent NFS-60 cell line and adaption to growth in macrophage colony-stimulating factor. J. Immunol. 145: 860-864, 1990. PubMed: 2142710

23295: Weinstein Y, et al. Truncation of the c-myb gene by a retroviral integration in an interleukin 3-dependent myeloid leukemia cell line. Proc. Natl. Acad. Sci. USA 83: 5010-5014, 1986. PubMed: 3014527



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
慈利县| 尤溪县| 板桥市| 通化县| 渑池县| 沧州市| 花垣县| 肃宁县| 和平区| 岑溪市| 孟连| 涞源县| 灌云县| 汝州市| 体育| 闻喜县| 兴海县| 乌鲁木齐县| 广水市| 公主岭市| 竹溪县| 丽水市| 曲沃县| 高陵县| 天长市| 渭源县| 施秉县| 乌审旗| 汝南县| 天祝| 项城市| 呼玛县| 吉木乃县| 高唐县| 红桥区| 东城区| 康平县| 康保县| 石景山区| 浦东新区| 麻栗坡县|